Rodina M ; Cosson J ; Gela D ; Linhart O ;

Aquaculture International vol. 12 (1)   pp. 119-131, 2004

Joint Laboratory of Genetics, Physiology and Reproduction of Fishes, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic and University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic

This study tested KUROKURA solution and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of potential motility of tench spermatozoa. The immobilizing solution is used because, when collected, the sperm of most samples is contaminated by urine, causing spermatozoa to be of poor quality, with low motilities and velocities (almost 0), thus resulting in a worsened fertilization and hatching rate. Sperm was sampled with a syringe containing an immobilizing solution (IS), allowing an IS:sperm ratio of 2:1, under aerobic conditions at 0-4 degree C. This sperm solution was stored for 10 h and untreated sperm was collected prior to fertilization as a control. Spermatozoa quality was evaluated for the cell motility and velocity parameters and also for fertilization ability and hatching rate. Results obtained for tench sperm motility, velocity, fertilization and hatching rate showed that only sperm collected in the various immobilizing solutions can be successfully used for artificial insemination and preservation after 10 h at 0-4 degree C. The best immobilizing solution was found to be KUROKURA 180 (180 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl sub(2). 2H sub(2)O and 2.38 mM NaHCO sub(3)), giving a fertility and hatching rate of 41%, with no change in rates after 10 h storage of sperm. Control sperm without immobilizing solution showed a fertilization and hatching rate of only 6-7%

Sperm ;  Hatching ;  Fertilization ;  Preservation ;  Fertility ;  Aerobic conditions ;  Cell migration ;  Artificial insemination ;  Tinca tinca ; 

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