Urbanyi B ; Horvath A ; Kovacs B ;

Aquaculture International vol. 12 (1)   pp. 47-56, 2004


urbanyib@nt.ktg.gau.hu

Successful hybridization of sterlet (Acipenser ruthenus) x Siberian sturgeon (Acipenser baeri), sterlet x Russian sturgeon (Acipenser gueldenstaedti) and sterlet x European sturgeon (Acipenser sturio) was carried out for the first time using cryopreserved semen of sturgeon males and sterlet x sterlet crosses as controls. Sperm of all three species was diluted with a cryodiluent composed of 23.4 mM sucrose, 0.25 mM KCl, 30 mM Tris (pH 8.0) and 10% methanol. The samples were frozen in plastic straws in the vapor of liquid nitrogen at the height of 3 cm above the level of nitrogen for 3 min. Following thawing approximately 3000 sterlet eggs were fertilized with six straws of frozen-thawed sperm. The hatching rate with sterlet sperm was 30.6% while the hatching rate of A. ruthenus x A. baeri, A. ruthenus x A. gueldenstaedti and A. ruthenus x A. sturio hybrids was 50, 17.4 and 34%, respectively. Morphometric markers as well as random amplified polymorphic DNA (RAPD) assay was used to verify interspecific hybridization

Sperm ;  Hatching ;  Cryopreservation ;  Nitrogen ;  Hybrids ;  Plastics ;  Interspecific hybridization ;  Semen ;  Polymerase chain reaction ;  Methanol ;  Random amplified polymorphic DNA ;  Eggs ;  Thawing ;  Acipenser sturio ;  Acipenser gueldenstaedti ;  Acipenser ruthenus ; 

Consulter l'article complet ...