Antonio DB ; El-Matbouli M ; Hedrick RP ;

Parasitol Res vol. 85 (11)   pp. 942-4, Nov 1999

School of Veterinary Medicine, Department of Medicine and Epidemiology, University of California, Davis 95616, USA.

The myxosporean and actinosporean spores of Myxobolus cerebralis develop through many stages in their respective hosts, salmonid fishes and a tubificid oligochaete. Using a modified, non-radioactive in situ hybridization protocol, the parasite, which exhibits radically different structural forms during its development in each host, could be specifically detected in paraffin-embedded tissues of both fish and oligochaetes. Our study aims to demonstrate the application of the technique for detection of early stages of M. cerebralis in both hosts.

Animal ;  Fish Diseases: parasitology ;  Host-Parasite Relations ;  In situ hybridization ;  Oligochaeta: parasitology ;  Oncorhynchus mykiss: parasitology ;  Protozoa: growth & development: isolation & purification ;  Protozoan Infections,Animal: parasitology ;  Specific Pathogen-Free Organisms ;  1130 ;