Daniels GD ; Zou J ; Charlemagne J ; Partula S ; Cunningham C ; Secombes CJ ;

J Leukoc Biol vol. 65 (5)   pp. 684-90, Mai 1999

Department of Zoology, University of Aberdeen, Scotland, United Kingdom.

Two rainbow trout chemokine receptors have been sequenced, with homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a mature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7. Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors. Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and CXC-R1-2. Reverse transcriptase- polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.

Amino acid sequence ;  Animal ;  Cattle ;  Cloning,Molecular ;  Human ;  Mice ;  Molecular Sequence Data ;  Oncorhynchus mykiss: immunology ;  Organ Specificity: immunology ;  Phylogeny ;  Rats ;  Receptors,CXCR4: genetics: isolation & purification ;  Receptors,Chemokine: genetics: isolation & purification ;  Reverse Transcriptase Polymerase Chain Reaction ;  Sequence Homology,Amino Acid ;  Support,Non-U.S.Gov't ;  18530 ;