De Craemer D ; Vamecq J ; Roels F ; Vallee L ; Pauwels M ; Van den Branden C ;

J Lipid Res vol. 35 (7)   pp. 1241-50, Jui 1994

Menselijke Anatomie & Embryologie, Vrije Universiteit Brussel, Belgium.

Male NMRI mice were fed a diet with 10% w/w Beromegan for up to three weeks. Beromegan is a commercial fish (salmon) oil preparation rich in eicosapentaenoic acid and docosahexaenoic acid. Peroxisomal beta- oxidation capacity, catalase activity, and ultrastructural morphometry of the hepatic peroxisomes were investigated. In myocardium and kidney, catalase activity, peroxisomal staining after catalase cytochemistry, peroxisomal morphology, and morphometry (in myocardium) were evaluated. In liver, we found a significant increase in peroxisomal beta- oxidation, catalase activity, and peroxisomal number already after 3 days of dietary treatment. These changes were more pronounced after 3 weeks. Peroxisomal size was not changed. Positive correlations were found between peroxisomal enzyme activities and the number but not the size of the peroxisomes, and between catalase activity and beta- oxidation capacity. The mean peroxisomal diameter per animal was inversely proportional to catalase activity measured in homogenate. In myocardium, catalase activity was increased with duration of fish oil feeding. Peroxisomal staining, number, and size were also increased when compared to controls. In kidney, no alterations were observed. Our results indicate a beneficial effect of a diet supplemented with fish oil on the peroxisomal metabolism in liver and myocardium; it differs from the changes induced by xenobiotic peroxisome proliferation.

Animal ;  Comparative Study ;  Dietary Fats,Unsaturated: pharmacology ;  Fish Oils: pharmacology ;  Kidney: enzymology: ultrastructure ;  Liver: enzymology: ultrastructure ;  Male ;  Mice ;  Mice,Inbred Strains ;  Microbodies: enzymology: ultrastructure ;  Myocardium: enzymology: ultrastructure ;  Organ Weight: physiology ;  Random Allocation ;  Salmon ;  Species Specificity ;  Support,Non-U.S.Gov't ;  33630 ;