Hung SS ; Cho CY ; Slinger SJ ;

J Nutr vol. 111 (4)   pp. 648-57, Avr 1981

A factorial experiment was conducted using two degrees of oxidation of the 7.5% supplemental fish oil (peroxide values of 5 and 120 meq/kg oil), two levels of supplemental DL-apha-tocopheryl acetate (0 and 33 mg/kg diet) and two levels of ethoxyquin (0 and 125 mg/kg diet) supplementation. Dietary thiobarbituric acid number, weight percentage of polyunsaturated fatty acids and omega-three fatty acids in the total fatty acids were significantly (P less than 0.05) different between diets with fresh and highly oxidized oil. Dietary RRR-alpha-tocopherol was significantly (P less than 0.05) reduced by the addition of highly oxidized oil after 24 weeks storage of the feed while supplemental DL- alpha-tocopheryl acetate level was not changed. Fish fed the various diets showed no differences in growth, feed:gain ratio, carcass composition or plasma glutathione peroxidase activity. The mortality, percent red cells hemolyzed by hydrogen peroxide, plasma and liver RRR- alpha-tocopherol concentrations were significantly (P less than 0.05) affected by the addition of highly oxidized oil or DL-alpha-tocopheryl acetate but not by ethoxyquin except that mortality was reduced by supplementary ethoxyquin. The results of this study suggested that no vitamin E or ethoxyquin supplementation was needed to prevent a deficiency of vitamin E in rainbow trout fed a practical diet containing 7.5% of a good quality herring oil for 24 weeks.

Animal ;  Animal nutrition ;  Diet ;  Drug Stability ;  Ethoxyquin: administration & dosage ;  Fish Oils: administration & dosage ;  Hemolysis: drug effects ;  Liver: metabolism ;  Oxidation-Reduction ;  Quinolines: administration & dosage ;  Salmonidae: physiology ;  Support,Non-U.S.Gov't ;  Trout: physiology ;  Vitamin E: analogs & derivatives: metabolism: physiology ;  64460 ;