Miller KG ; Wendland B ; Scheller RH ;

Brain Res vol. 616 (1-2)   pp. 99-104, 9 Jui 1993

Howard Hughes Medical Institute, Beckman Center for Molecular and Genetic Medicine, Department of Molecular and Cellular Physiology, Stanford University Medical Center, CA 94305-5428.

In this study, we used synaptic vesicles purified from the electric organ of marine electric rays to search for novel molecules which have important functions in synaptic transmission. Proteins that copurified with synaptic vesicles were used to immunize rats, and the resulting antisera were then used to further characterize the vesicle proteins. One of the antisera recognizes a protein of 34 kDa, p34, that has several characteristics which suggest it is a synaptic vesicle specific protein: (1) it copurifies exclusively with the synaptic vesicle peak during permeation chromatography on a controlled pore glass beads column, (2) it can be immunoprecipitated with intact synaptic vesicles and (3) it is specifically localized to the nervous system. The results suggest that p34 is a synaptic vesicle specific protein with a widespread distribution in the nervous system.

Amino acid sequence ;  Animal ;  Biological Markers: analysis ;  Blotting,Western ;  Brain Chemistry ;  Cell Fractionation: methods ;  Comparative Study ;  Electric Organ: chemistry ;  Electrophoresis,Polyacrylamide Gel ;  Liver: chemistry ;  Molecular Sequence Data ;  Molecular weight ;  Muscles: chemistry ;  Nerve Tissue Proteins: analysis: chemistry: isolation & purification ;  Organ Specificity ;  Sequence Homology,Amino Acid ;  Skates (Fish) ;  Spinal Cord: chemistry ;  Support,U.S.Gov't,P.H.S. ;  Synaptic Vesicles: chemistry ;  36250 ;