Perdu-Durand EF ; Tulliez JE ;

Food Chem Toxicol vol. 23 (3)   pp. 363-6, Mar 1985

The in vitro metabolism of n-heptadecane was investigated using hepatic microsomes from Hubbard chickens, New Zealand rabbits, Wistar rats and rainbow trout. Incubations with the 14C-labelled alkane for 1 hr showed that the rate of oxidation varied between species; the rate (per mg protein) in chickens was roughly 20-fold greater than the rate in trout, and roughly 10-fold greater than the rates in rats and rabbits. On the basis of cytochrome P-450 content, the rate of heptadecane metabolism was roughly 20-fold greater in the chicken than in the other species. Moreover, the lambda max Soret band of the reduced cytochrome P-450 CO-complex was observed at 452 nm in the chicken. Correlation between the rate of heptadecane metabolism and in vivo storage levels is discussed.

Alkanes: metabolism ;  Animal ;  Chickens ;  Chromatography,Thin Layer ;  Comparative Study ;  Cytochrome P-450: metabolism ;  Hydrocarbons: metabolism ;  Hydroxylation ;  In vitro ;  Male ;  Microsomes,Liver: enzymology: metabolism ;  Rabbits ;  Rats ;  Rats,Inbred Strains ;  Species Specificity ;  Time Factors ;  Trout ;  54730 ;