Aquaculture

The applicability of large-scale sperm cryopreservation in wels catfish (Silurus glanis) optimized for hatchery practice

Publication date: 15 May 2019

Source: Aquaculture, Volume 506

Author(s): Zoltán Bokor, Gergely Bernáth, Levente Várkonyi, József Molnár, Zete Levente Láng, Zsófia Tarnai-Király, Enikő Solymosi, Béla Urbányi

Abstract

In the presented study, effects of sperm cryopreservation methods (Polystyrene box-P. box vs a controlled-rate freezer-CRF) and various semen confection methods during freezing (5 mL straw vs. 10 mL cryotube) on sperm motility parameters and fertilization capacity of wels catfish (Silurus glanis) were tested. In general, pMOT (Control: 89 ± 3%, Straw P. box: 50 ± 9%, Straw CRF: 53 ± 12%, Cryotube: 52 ± 7%) and BCF (Control: 30 ± 1 Hz, Straw P. box: 27 ± 1 Hz, Straw CRF: 26 ± 1 Hz, Cryotube: 27 ± 1 Hz) showed significant reduction following thawing. ALH reduced significantly in the case of the straw frozen with P. box (1.2 ± 0.2 μm) compared to the fresh control (1.5 ± 0.1 μm). However, VCL (Control: 108 ± 6 μm/s, Straw P. box: 107 ± 5 μm/s, Straw CRF: 108 ± 8 μm/s, Cryotube: 109 ± 7 μm/s) and VSL (Control: 94 ± 6 μm/s, Straw P. box: 99 ± 4 μm/s, Straw CRF: 99 ± 9 μm/s, Cryotube: 99 ± 7 μm/s) did not decrease. A significantly higher LIN was measured using the straw cryopreserved in the P. box (92 ± 2%) and the CRF (91 ± 2%) in comparison with the fresh control (86 ± 1%). No significant difference in the fertilization capacity when eggs were fertilized by frozen/thawed (Straw P. box: 75 ± 5%, Straw CRF: 72 ± 3%, Cryotube: 66 ± 6%) or fresh (control, 68 ± 4%) semen was observed (P > .05). As well, no significant correlation between none of sperm motility parameters tested and the fertilization rate were recorded (P > .05). All tested sperm cryopreservation showed a similar high efficiency in the hatchery practice of wels catfis.

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