Developmental & Comparative Immunology

The host defense peptide β-defensin confers protection against Vibrio anguillarum in ayu, Plecoglossus altivelis.

The host defense peptide β-defensin confers protection against Vibrio anguillarum in ayu, Plecoglossus altivelis.

Dev Comp Immunol. 2019 Sep 30;:103511

Authors: Zhou Y, Zhou QJ, Qiao Y, Chen J, Li M

Abstract
β-defensin is a cationic host defense peptide actively participating in host innate immune response against pathogens. In teleost fish, β-defensin exhibits a diversity in genotypes and functions. Herein, a β-defensin homolog (PaBD) was identified from ayu, Plecoglossus altivelis, showing multiple tissues' upregulation against Vibrio anguillarum challenge. In vivo experiments revealed that intraperitoneal injection of chemically synthesized mature PaBD (mPaBD) increased the survival rate of V. anguillarum-infected ayu, accompanied by reduced bacterial load and decreased tissue mRNA level of tumor necrosis factor α (PaTNF-α) and interleukin 1β (PaIL-1β). However, in vitro, mPaBD showed weak bactericidal activity against V. anguillarum. Interestingly, mPaBD enhanced phagocytosis, intracellular bacterial killing, and respiratory burst of ayu monocytes/macrophages (MO/MΦ). Moreover, it inhibited mRNA level of PaIL-1β and PaTNF-α in MO/MФ upon V. anguillarum infection. In conclusion, PaBD protects ayu against V. anguillarum challenge not only through its direct antibacterial ability, but also through its immunomodulation in MO/MΦ.

Glossaire technique

Expérimentation biologique qui se fait à l'extérieur de l'organisme (ex....
Manifestation pathologique due à l'action des microbes.
Nom courant désignant les poissons de la famille des Cyprinodontidés...
Dérivant des monocytes, les macrophages sont capable de phagocytose. Il...
Variété de globules blancs. Les monocytes circulent dans le sang pour se...
Diminutif du nom vernaculaire Plecostomos (Hypostomus plecostomus). Ce...