Developmental & Comparative Immunology

ITLN in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var ♂) is involved in host defense against bacterial infection.

ITLN in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var ♂) is involved in host defense against bacterial infection.

Dev Comp Immunol. 2019 Oct 15;:103520

Authors: Luo SW, Luo KK, Liu SJ

Abstract
Hybrid genotypes in fish may be less susceptible to pathogenic infection. ITLN, a novel lectin, not only exhibits a regulatory role in pathogenic infection, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-ITLN was 945 bp and encoded 314 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-ITLN was observed in liver. Aeromonas hydrophila challenge can sharply increased WR-ITLN mRNA expression in liver, kidney and spleen. The purified WR-ITLN protein can directly bind to A. hydrophila and S. agalactiae, reduce their relative bacterial activity and limit bacterial growth in vitro in the presence of Ca2+. In addition, the treatment of WR-ITLN + Ca2+ can restrict bacterial dissemination in vivo and attenuate production of pro-inflammatory cytokines. These results indicated that WR-ITLN can confer protection against bacteria-stimulated MyD88-dependent pro-inflammatory cytokines activation in a Ca2+-dependent manner.

Glossaire technique

Substance sécrétée par les lymphocytes (globules blancs) et les...
Expérimentation biologique qui se fait à l'extérieur de l'organisme (ex....
Manifestation pathologique due à l'action des microbes.
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