Fish & Shellfish Immunology

Functional analysis of the CXCR1a gene response to SGIV viral infection in grouper

Publication date: May 2019

Source: Fish & Shellfish Immunology, Volume 88

Author(s): Qing Wang, Min Yang, Chen Li, Shaowen Wang, Yuxin Wang, Fangmei Lin, Leyun Zheng, Yepin Yu, Qiwei Qin

Abstract

Chemokine receptors are a superfamily of seven-transmembrane domain G-coupled receptors and have important roles in immune surveillance, inflammation, and development. In previous studies, a series of CXCRs in grouper (Epinephelus coioides) was identified; however, the function of CXCR in viral infection has not been studied. To better understand the effect of the CXCR family on the fish immune response, full-length CXCR1a was cloned, and its immune response to Singapore grouper iridovirus (SGIV) was investigated. Grouper CXCR1a shared a seven-transmembrane (7-TM) region and a G protein-coupled receptor (GPCR) family 1 that contained a triaa stretch (DRY motif). Phylogenetic analysis indicated that CXCR1a showed the nearest relationship to Takifugu rubripes, followed by other fish, bird and mammal species. Fluorescence microscopy revealed that CXCR1a was expressed predominantly in the cytoplasm. Overexpression of CXCR1a in grouper cells significantly inhibited the replication of SGIV, demonstrating that CXCR1a delayed the occurrence of cytopathic effects (CPE) induced by SGIV infection and inhibited viral gene transcription. Furthermore, our results also showed that CXCR1a overexpression significantly increased the expression of interferon-related cytokines and activated ISRE and IFN promoter activities. Taken together, the results demonstrated that CXCR1a might have an antiviral function against SGIV infection.

Glossaire technique

Substance sécrétée par les lymphocytes (globules blancs) et les...
Propriété d'un composé à réémettre de l'énergie sous forme lumineuse.
Le genre Takifugu est un genre comprenant des poissons appelés fugu,...
Manifestation pathologique due à l'action des microbes.
Réaction de l'organisme en réponse à une lésion tissulaire. Ce mécanisme...
Mince couche de tissu couvrant une partie d'un animal ou reliant les...
Relatif à un virus.
Entité biologique nécessitant un hôte, souvent une cellule, dont il...
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