Fish & Shellfish Immunology

Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection

Publication date: December 2019

Source: Fish & Shellfish Immunology, Volume 95

Author(s): Xue-peng Li, Shuai Jiang, Bin Sun, Jian Zhang

Abstract

Interleukin-10 (IL-10) is a pleiotropic cytokine and plays a crucial role in immunity. In the current study, we examined the expression patterns and biological functions of tongue sole Cynoglossus semilaevis IL-10 (CsIL-10). CsIL-10 is composed of 186 amino acid residues and shares 46.3%–71.7% identities with other teleost IL-10. Csil-10 expression occurred in multiple tissues and was regulated by bacterial infection. Recombinant CsIL-10 (rCsIL-10) in the form of a dimer bound to a wide range of bacterial species but did not affect bacterial growth. rCsIL-10 could interact with peripheral blood leukocytes (PBL) and significantly reduce the phagocytic activity, ROS production, and apoptosis of PBL. When injected in vivo, rCsIL-10 significantly suppressed the expression of proinflammatory cytokines and promoted bacterial dissemination in tongue sole tissues. Consistently, knockdown of Csil-10 significantly inhibited bacterial infection in tongue sole. Taken together, these results indicate that CsIL-10 plays a negative regulatory role in the immune response against bacterial infection.

Glossaire technique

Substance sécrétée par les lymphocytes (globules blancs) et les...
Manifestation pathologique due à l'action des microbes.